Overview of antibody use in immunocytochemistry.
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Production and characterization of polyclonal antibody against a synthetic peptide from β-actin protein
Objective(s):Antibodies against actin, as one of the most widely studied structural and multifunctional housekeeping proteins in eukaryotic cells, are used as internal loading controls in western blot analyses. The aim of this study was to produce polyclonal antibody against a synthetic peptide derived from N-terminal region of β-actin protein to be used as a protein loading control in western ...
متن کاملاندازه گیری ثابت تمایل (افینیتی) آنتی بادی مونوکلونال IgG ضد آنزیم الکالین فسفاتاز با استفاده از روش الیزا
Background and Aim: The affinity of an antibody with its antigen is a crucial parameter in its biological activity and carrying out immunologic tests such as radioimmunoassay (RIA) and immunohistochemistry. This study was done in our laboratory at Tarbiat Modarres University to measure the affinity constant of specific monoclonal antibodies/MAB (A1G8F7) with alkaline phosphatase enzyme produced...
متن کاملبررسی اثر لیتیوم کلراید در القای سلولهای استرومایی مغز استخوان به سلولهایی با فنوتیپ عصبی
Background & Objective : Bone marrow stromal cells (BMSCs) are a kind of stem cells with high pluripotency. The BMSCs can differentiate into mesodermal and non mesodermal cells. Because of availability of them, they are a suitable source of adult stem cells for cell therapy. Some inducers were used to differentiate stem cells into neural phenotype, such: retinoic acid, dimethyl sulfoxide, dep...
متن کاملPolyclonal Antibody against Recombinant Nucleoprotein of the Influenza A Virus (H1N1); Production and Purification
Background and Aims: Influenza is an acute respiratory illness that is caused by a virus belonging to Orthomyxoviridae family. This virus spreads rapidly every year in cold season and leads to morbidities and mortalities especially in adults and children, which causes billions of dollars of economic losses. Accordingly, development of a rapid, sensitive and inexpensive laboratory diagnosis base...
متن کاملMonoclonal and Polyclonal Antibodies Specific to Human Fibromodulin
Background: The unique expression of fibromodulin (FMOD) in patients with chronic lymphocytic leukemia (CLL) has been previously reported. Detecting FMOD in CLL patients using specific anti-FMOD mAbs might provide a promising method in detection, monitoring, and prognosis of CLL. Objectives: In this study, we aimed for producing specific antibodies agains...
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عنوان ژورنال:
- Methods in molecular biology
دوره 34 شماره
صفحات -
تاریخ انتشار 1994